Calculate the initial concentration before dilution.

Initial Concentration: M

A reverse dilution calculator is a tool used to determine the initial concentration of a solution before it was diluted to a known final concentration. This can be useful in various scientific and laboratory applications where you need to understand the starting concentration of a substance based on the dilution factor and the final concentration.

Here’s how a reverse dilution calculator works and its formula:

Formula for Reverse Dilution:

The formula for calculating the initial concentration (C1) before dilution is as follows:

C1 = (C2 * V2) / V1


  • C1 = Initial concentration (before dilution)
  • C2 = Final concentration (after dilution)
  • V1 = Volume of the initial solution (before dilution)
  • V2 = Volume of the solvent or diluent added

Steps to Use the Reverse Dilution Calculator:

Gather Information:

  • You need to know the final concentration (C2) of the diluted solution.
  • Measure the volume of the diluted solution (V2) after dilution.
  • Determine the volume of the solvent or diluent (V1) added during the dilution process.

Plug the Values into the Formula:

Insert the values you gathered into the formula mentioned above.

Calculate the Initial Concentration (C1):

Once you have all the values plugged into the formula, perform the calculation to find the initial concentration (C1).

Interpret the Result:

The result (C1) will represent the initial concentration of the solution before dilution.

Example: Suppose you have a 100 ml solution with a final concentration of 20 mg/ml, and you diluted it with an additional 100 ml of solvent. To find the initial concentration:

C2 (Final concentration) = 20 mg/ml V1 (Volume before dilution) = 100 ml V2 (Volume after dilution) = 100 ml

Using the formula: C1 = (C2 * V2) / V1 C1 = (20 mg/ml * 100 ml) / 100 ml C1 = 2000 mg/ml

So, the initial concentration of the solution before dilution was 2000 mg/ml.

Note For Reverse Dilution:

  • Ensure that all units are consistent (e.g., both volumes in milliliters and concentrations in milligrams per milliliter) when using the formula.
  • Be cautious about units and conversions when using the formula to avoid errors.

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